StudentShare
Contact Us
Sign In / Sign Up for FREE
Search
Go to advanced search...
Free

SOP of Nitrogen Purging of the Agilent Carey 5000 UV-Vis NIR Spectrophotometer - Research Paper Example

Cite this document
Summary
The purpose of this procedure is to ensure all necessary instructions/steps are taken for a safe and environmentally friendly, Nitrogen purging of the Agilent caret 5000 UV-Vis NIR spectrophotometer, without compromising the high standards of the eventual output. These…
Download full paper File format: .doc, available for editing
GRAB THE BEST PAPER97.4% of users find it useful
SOP of Nitrogen Purging of the Agilent Carey 5000 UV-Vis NIR Spectrophotometer
Read Text Preview

Extract of sample "SOP of Nitrogen Purging of the Agilent Carey 5000 UV-Vis NIR Spectrophotometer"

STANDARD OPERATING PROCEDURE Purpose: The purpose of this procedure is to ensure all necessary instructions/steps are taken for a safe and environmentally friendly, Nitrogen purging of the Agilent caret 5000 UV-Vis NIR spectrophotometer, without compromising the high standards of the eventual output. These instructions are meant to ensure: health of the operators is not put at any risk and that they have adequate knowledge to undertake the process; procedures go on in a systematic manner without failures.

(Mannan, 2005)Scope of this SOP:The scope of this Sop covers the Nitrogen purging of the Agilent Cary 5000 UV-Vis NIR spectrometry. Explicit equipment levels, rules, method and declarations shall be established before the work being undertaken. These shall comprise the Scope of Work. (Brian, 2006)ProcedureA) Operator-Production supervisor-Selects the equipment to be used and reviews the methods to be deployed.-Fabrication Operator- Assembles all apparatus, materials (gases) and equipment, having tested them respectively.

Should ensure perforated trays are well placed and all set-ups are perfect.B) OH&S Information-The operator(s) of the purge process should be in protective clothing, sober mind and completely masked from the contact with possible electromagnetic radiations. They should be trained to be able to procure first aid services. (Patricia, Ronald & Richard, 2002)- Procedure should be done in a spacious environment with a first aid kit strategically placed. A well labeled emergency exit and trash bin should be in place (John, 1998) pg. 75C) Equipment and Materials:Precaution: Nitrogen should never be flushed without valves.

All cylinders must have air tight valves. (Brian, 2006)1. Nitrogen source set up2. Plastic supply tubing (to direct the nitrogen jet)3. Pressure regulator and gauge 4. Gas manifold 5. Agilent Cary 5000 UV-Vis NIR spectrophotometer6. Sample compartment7. Heat exchanger (heat regulation)8. Flow Meters9. Screw drivers(to undo the plates and panels)10. Ultra-pure liquefied Nitrogen (5.0)D) Instructions1. Make sure all environmental hazards are checked, and precautions highlighted herein are taken into consideration.2. Ensure the Nitrogen purge system is set up to ensure constant nitrogen supply3.

Fit the extended compartment to the spectrophotometer.(note the spectrophotometer should not be connected to a mains power source)4. Connect the tubing from the nitrogen purge system underneath the inlet tubes of the extended sample compartment.5. Open the nitrogen control valve to flush the Nitrogen at a supply pressure of between 83-172kPA6. Adjust the array of flow meters ensure optimum circulation conditions are met.a. Instrument:0-20 l/minb. Sample compartment:0-10 l/min7. Allow the Nitrogen purging to run for 20minutes the close the nitrogen supply control valve8.

Check the extend of purging by doing an oxygen scan and water vapor at the U.V-vis region and NIR region respectively.(Sam, 2005)9. Once satisfied with the extend, detach the sample compartment from the nitrogen supply system (Caldwell, 1998)10. Remove the base of the sample compartment from the spectrophotometer 11. Realign the optics with respect to the floor of the spectrophotometer12. For safe removal of the base please ensure you: a. Remove the front doorb. Remove the cover plate between the horizontal extrusion (unscrew the two screws at its base) and floorc.

Remove the lower plated. Undo the fasteners holding the sample compartments floor and remove it.E) The production operator notes all parameters measured (water favor levels, oxygen levels, optics alignments) and along with other documents and forwards them to the supervisor.F) The supervisor inspects the work done and signs relevant logs, records and audit sheets.References,Brian, J. G. (2006). Hazardous Waste Operations and Emergency Response Manual. New Jersey: john wiley and sons.Caldwell, Marissa Anne;. (2011). Colloidal Nanoparticles for Phase Change Memory Applications (I ed., Vol. One). (C. E.

Dunn chidsey, D. J. Milliron, & H.-s. P. Wong, Eds.) Stanford, California, USA: Stanford University.George, P. D., Hill, R. H., & Barth, R. C. (2003). Environmental Health and Safety for Hazardous Waste Sites (illustrated ed.). (P. D. George, H. R. Hill, & R. C. Barth, Eds.) Geneva: AIHA.John, L. C. (1998). Standard Operating Procedures and Guidelines. Tulsa,Oklahoma: PennWell Books.Sam, M. (2005). Lees Loss Prevention in the Process Industries: Hazard Identification. Los Angeles: Butterworth-Heinemann.

Read More
Tags
Cite this document
  • APA
  • MLA
  • CHICAGO
(“SOP of Nitrogen Purging of the Agilent Carey 5000 UV-Vis NIR Research Paper”, n.d.)
SOP of Nitrogen Purging of the Agilent Carey 5000 UV-Vis NIR Research Paper. Retrieved from https://studentshare.org/chemistry/1660303-sop-of-nitrogen-purging-of-the-agilent-carey-5000-uv-vis-nir-spectrophotometer
(SOP of Nitrogen Purging of the Agilent Carey 5000 UV-Vis NIR Research Paper)
SOP of Nitrogen Purging of the Agilent Carey 5000 UV-Vis NIR Research Paper. https://studentshare.org/chemistry/1660303-sop-of-nitrogen-purging-of-the-agilent-carey-5000-uv-vis-nir-spectrophotometer.
“SOP of Nitrogen Purging of the Agilent Carey 5000 UV-Vis NIR Research Paper”, n.d. https://studentshare.org/chemistry/1660303-sop-of-nitrogen-purging-of-the-agilent-carey-5000-uv-vis-nir-spectrophotometer.
  • Cited: 0 times

CHECK THESE SAMPLES OF SOP of Nitrogen Purging of the Agilent Carey 5000 UV-Vis NIR Spectrophotometer

Oral Controlled Release System of Propranolol

Briefly, each dilution placed on a clear cuvette was loaded into the spectrophotometer (fig.... Ionotropic gelation is based on the ability of polyelectrolytes such as gellan gum to cross link in the presence of counter ions, like Calcium (Ca2+) and Zinc (Zn3+) ions, to form hydrophilic hydrogel beads (gelispheres)....
6 Pages (1500 words) Dissertation

The Concept of Molecular Food Microbiology

Health & Safety Requirements Submitted to: [Pick the date] Health & Safety Requirement of Food Microbiology Food microbiology concerns with the study of microorganisms that have a tendency of affecting the quality and overall nature of food by making it contaminated.... hellip; Whereas the subject is rather vast itself, there are several health and safety requirements to be fulfilled while examining food for microorganisms uniformly....
4 Pages (1000 words) Essay

Physics in The Atmospheric Chemistry Experiment

The satellite operated in the UV-Visible-NIR region of the spectrum with the ACE-FTS being used in the NIR region while the MAESTRO spectrophotometer was being used over the UV-VIS-NIR region (ACE, 2009).... the MAESTRO spectrophotometer operated in the UV-VIS-NIR spectral region of 285-1030nm with a vertical resolution of 1-2 km....
4 Pages (1000 words) Research Paper

Determining Nitrous Oxide Content in Gases

The gas has many industrial uses and is also naturally emitted during the nitrogen cycle.... Nitrous oxide, popularly known as the laughing gas has been used in medicine as an anaesthetic since the late 18th century and also misused for recreational purposes.... hellip; The use of fertilizers in agriculture, pollution and other human interventions has resulted in increased concentrations of the gas in the atmosphere and work places that has instilled fear for environmental /health safety. In this context, monitoring Nitrous oxide concentrations becomes pertinent....
5 Pages (1250 words) Essay

Determination of nitrogen dioxide content of the atmosphere

The author of this lab report "Determination of nitrogen dioxide content of the atmosphere" comments on the Griess-Saltzman Reaction which determines the nitrogen dioxide content of the atmosphere.... nbsp;… The method is used for manual determination of nitrogen dioxide in the atmosphere present in the range of 0.... Apparatus include sampling probe, absorber, gas drying tube, air-metering device, thermometer, manometer, air pump, spectrophotometer, and stopwatch....
7 Pages (1750 words) Lab Report

Spectrophotometer & Immunochemistry

The paper "spectrophotometer & Immunochemistry" states that there are different versions of a spectrophotometer, and on the other hand, there are different examples of immunoassays, such as radioimmunoassay, fluorescence immunoassay, etc (Geddes, pp.... Although it is a creation of physics, however, it is an observation that a broad range of fields has been beneficial of this capability of a spectrophotometer, such as biochemistry, molecular biology, etc....
8 Pages (2000 words) Coursework

Measuring Metabolites in Plasma Samples by Flame Photometry and Spectrophotometry

urplus nitrogen in the body is removed through The formation of urea and its eventual excretion by the kidneys (~75%).... The dependence of plasma urea on the rate of formation-nitrogen turnover and the rate of its excretion is a limitation in its use.... This work "Measuring Metabolites in Plasma Samples by Flame Photometry and Spectrophotometry" focuses on the concentration of plasma urea and creatinine....
6 Pages (1500 words) Research Paper

Estimation of Protein Concentration by Spectrophotometry

This lab report "Estimation of Protein Concentration by Spectrophotometry" presents spectrophotometry as a standard technique for measuring the concentration in a solution of a substance that absorbs light, since the degree of absorption is proportional to the concentration of the solute.... hellip; In the case of proteins, a solution is allowed to react with the Biuret reagent (a combination of a base such as potassium hydroxide and copper II sulfate); the copper forms a complex with the peptide bonds of the protein and the resulting solution has a strong purple color, with maximum light absorption at a wavelength of 540 nm....
7 Pages (1750 words) Lab Report
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Contact Us