StudentShare
Contact Us
Sign In / Sign Up for FREE
Search
Go to advanced search...
Free

Factors to Be Considered in Conducting Plasmid Purification - Term Paper Example

Cite this document
Summary
This term paper "Factors to Be Considered in Conducting Plasmid Purification" focuses on an area of microbiology that involves some principles which in most cases are performed in laboratories. In this particular case what is of interest is to do with the purification of DNA. …
Download full paper File format: .doc, available for editing
GRAB THE BEST PAPER95.4% of users find it useful
Factors to Be Considered in Conducting Plasmid Purification
Read Text Preview

Extract of sample "Factors to Be Considered in Conducting Plasmid Purification"

The paper "Factors to Be Considered in Conducting Plasmid Purification" is an excellent example of a term paper on biology. There are various ways through which DNA purification can be done and plasmid purification happens to be an example of these. In most cases what happens when conducting plasmid purification is that there are some kits involved in the process.

These kits are what will help an individual to isolate the plasmid DNA on the basis of the scale and the purity that is needed. (Horak, 1998) In this regard, there are some things that are to be put into consideration when conducting plasmid purification. In this case, it will be of the essence to consider some of the things that are of importance when carrying out this process. Possession of a plasmid number This determines the volume of culture that will be used in the purification process.

Again in this same regard, it should be noted that manipulation of some of the properties of these plasmid patterns may influence the final outcome. Such properties include the size, origin of replication among other things. These will influence the outcome in terms of the quality and the DNA yield. The number attached to this will influence the binding capacity, for example, QUIGEN-tip 100 and QUIGEN-tip 500. The nature of the host bacteria The strain of the bacteria used will go a long way in influencing the quality and the quantity.

It has been seen that when high amounts of carbohydrates have released the quality of the DNA that is released will be comparatively lower. At the same time, high endonuclease levels during lysis will bring about low DNA quality. These are exhibited in HB101 and the JM100 series among others. The Background of the chosen bacteria There should be ambiotic selection in all aspects of growth. This ensures that the cells not receiving plasmid fails to continue to grow and thrive even under the pressures of ambiotic.

The sensitivity of these ambiotics to temperature changes is also another fact that should be taken into account. Application of Plasmid Purification Gene Therapy There are some gene therapies whose success is greatly dependent on the insertion of some genes which prove to be therapeutic and in this case without causing mutations, injury or an immune response. 

Read More
Cite this document
  • APA
  • MLA
  • CHICAGO
(Factors to Be Considered in Conducting Plasmid Purification and Its Term Paper, n.d.)
Factors to Be Considered in Conducting Plasmid Purification and Its Term Paper. https://studentshare.org/biology/1773381-plasmid-purification
(Factors to Be Considered in Conducting Plasmid Purification and Its Term Paper)
Factors to Be Considered in Conducting Plasmid Purification and Its Term Paper. https://studentshare.org/biology/1773381-plasmid-purification.
“Factors to Be Considered in Conducting Plasmid Purification and Its Term Paper”. https://studentshare.org/biology/1773381-plasmid-purification.
  • Cited: 0 times

CHECK THESE SAMPLES OF Factors to Be Considered in Conducting Plasmid Purification

The Process of Protein Purification

Because of the relative ease of bacterial replication and protein purification, it is thus a method of choice in conducting in depth studies of proteins.... here are factors to consider in doing protein purification.... Recent advancements in molecular biology have led to purification and characterization of proteins.... Recent advancements in molecular biology have led to purification and characterization of proteins.... Vaccines, gene therapy and replacement (such as that in insulin-deficiency) all have helped in improving health conditions, and have been based on good elucidation of structures, research on structure-function relationships, and establishment of protein purification specific to the amino acid sequence present....
10 Pages (2500 words) Literature review

Expression Of Recombinant Tick Histamine-Binding Protein

Future researches involve purification and characterization of the recombinant TC11485.... On the other hand, the HBP gene sequence used for this particular study was from cattle tick, Rhipicephalus microplus, which is considered to be an agricultural pest.... Histamine-binding proteins (HBP) are ubiquitous biomolecules with pharmacologic importance, since they have an immunosuppressing activity, which can help prevent adverse health conditions such as histamine shock....
30 Pages (7500 words) Lab Report

Marketing Planning of Mount Fran

External: Market Analyses Porter's Five Forces Analysis Rivalry among existing firms The industry could be considered as oligopoly.... Topic: Marketing Planning of Mount Franklin by developing the Situation analysis, Objectives and Strategy 1.... Company Introduction Coca-Cola Amatil Limited (CCA) is a large beverage and food & services company producing and marketing health and well being drinks among a host of other soft drinks, alcoholic drinks and food products across Australia and worldwide....
13 Pages (3250 words) Essay

The Challenges and Opportunities of the International Business Environment

Globalization is the biggest factor that has to be considered with regard to international business.... Other factors also make globalization a necessity in the long run.... In fact, the world, all competitors have the same opportunity and some traditional factors that used to be the factors determining who dominates the market will cease to be important....
8 Pages (2000 words) Coursework

Strain Gauge Calibration

The paper "Strain Gauge Calibration" states that for the purpose of applications, the calibration resistor is determined in order to come up with the same bridge output voltage that would be brought about when a strain gauge of the specified gage factor is exposed to a given strain.... ... ... ... As the object is being deformed, the foil is also experiencing deformation, causing a change in its electrical resistance....
5 Pages (1250 words) Lab Report

Product Design and the Cost of Production

The evaluation of commercial impacts, sales probability, innovative values, among other factors usually permits the firm to reinstate the final decision.... .... ... ... Product designIntroductionIn an endeavour to address the demands public for a portable hand washing dispenser, the company instigated two indispensable decisive decisions....
10 Pages (2500 words) Coursework

Directly Observe Sodium Chloride

Solution crystallization has been largely adopted as an industrial purification technique as well as a separation process in pharmaceuticals, textile and chemical industries.... The paper "Directly Observe Sodium Chloride" discusses that high concentrations of the sample solutions may take more than 24hours to saturate or fully dissolve especially at room temperature....
19 Pages (4750 words) Lab Report

The Liquid Biopsies and Traditional Histopathological Approaches

This paper ''The Liquid Biopsies and Traditional Histopathological Approaches'' tells that Histopathology has always been an important component of inpatient care.... The use of histopathology specimens not only improved the diagnosis of patients but helped shaped important decisions in the care and treatment of patients....
12 Pages (3000 words) Report
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Contact Us